Sensitive Glycoform Profiling of b-Interferon-1a (Avonex) and recombinant human Erythropoietin by CESI-TOF-MS Sensitive and high resolution glycoform profiling of intact rhEPO and Interferon (rhIFN-β) using CESI-MS with Neutral OptiMS Cartridge
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چکیده
130 protein-based therapeutics are licensed today. With the addition of anticipated new biotherapeutics, the market is forecasted to exceed $140 billion USD in 2017. These therapeutic drugs have changed and improved the quality of life of millions of people around the world in the past few decades. One of the most important quality attributes of a biopharmaceutical drug is its heterogeneity, which includes but is not limited to glycosylation patterns. Glycosylation may vary in degree and type, and is dependent on parameters such as cell line, host organism and cell culture conditions. During production, these variables can cause a broad spectrum of possible glycan species. Therefore the comprehensive analytical characterization of glycosylation heterogeneity is extremely important to ensure safety and efficacy of the biopharmaceutical drug.
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